Arvind等, 2012, 菊花预处理,表面灭菌,再生,伸长和驯化的流程标准化, 植物药与药理学杂志(online) Vol.1 No.8 pp.1-5 (doi: 10.5376/jpmp.cn.2012.01.0008)

Arvind et al., 2012, Standardization of Protocol for Pre-treatment, Surface Sterilization, Regeneration, Elongation and Acclimatization of Chrysanthemum morifolium Ramat, Zhiwuyao Yu Yaolixue Zazhi (online) Vol.1 No.8 pp.1-5 (doi: 10.5376/jpmp.cn.2012.01.0008)

目前的研究中，我们开发了一套体外再生专业流程，从泰国陈皇后菊花的舌状花以获得新的突变体。菊花舌状花使用代森锰锌-45 (0.1%)+多菌灵(0.1%)+ 8-HQC (200 mg/L)培养3小时，培养物的成活率最大(89.44%)。使用HgCl₂(0.1%)对舌状花进行表面杀菌4分钟，效果最好，成活率达91.04%。向MS培养基中添加4 mg/L BAP和0.1 mg/L NAA，再生率最高(93.33%)，每个外植体的微芽数最多(5.67)和芽再生所需天数最少(30.67天)。再生植株被转移到添加GA3 0.50 mg/L对于再生芽伸长最好，达30天。聚丙烯盖子的玻璃罐中1:1装上泥炭+组培土，将伸长的植物体转移其中，驯化成功。驯化25天后，完成植株体淬火，然后将植物转移到温室开花。

In the present study we have developed proficient in vitro regeneration protocol from the ray florets of chrysanthemum cv. Thai Chen Queen to isolate the novel mutants. The maximum survival percentage (89.44%) of cultures was found when the ray florets were per-treated with mancozeb-45 (0.1%) + carbendazim (0.1%) + 8-HQC (200 mg/L) for 3 hours. Culture establishment was found best (91.04%) when the ray florets surface sterilized with HgCl₂ (0.1%) for duration of four minutes. Murashige and Skoog (MS) media supplemented with 4 mg/L BAP and 0.1 mg/L NAA was found to the best for regeneration percentage (93.33%), number of microshoots per explants (5.67) and minimum days required for shoot regeneration (30.67). The regenerated plantlets were transferred on the MS media supplemented with GA3 0.50 mg/L was found to the best for shoot elongation after 30 days of elongation. The successful acclimatization of elongated plantlets was achieved by transferring them into glass jar with polypropylene cap filled with peat + soilrite (1:1). The hardening of plantlets was completed after 25 days of acclimatization and then plants were transferred to greenhouse for flowering.